Hosted by the US Environmental Protection Agency
Neurodevelopment is a period vulnerable to various insults, including those produced by environmental compounds, and an understanding of these effects continues to be needed. Although in vitro New Approach Methodologies (NAMs) are being developed to foster efficient screening for both neurotoxicity (NT) and developmental neurotoxicity (DNT), reliability of these methods for regulatory purposes is necessary. Currently there is indication of alignment of DNT NAMs with terms used in NT/DNT Adverse Outcome Pathways (AOPs) found on the Wiki site, as seen in key events such as “cell injury/death”, “decreased synaptogenesis”, or “decreased neuronal network function”. Here we aim to assess the unique protein signatures following developmental or acute exposures to known neurotoxicants in Long Evans rats, which can then be applied to an AOP framework as biological key events and further work to develop and show reliability of in vitro NAMs.